Bronchorelaxation Activity of Coptosapelta Flavescens Korth Root’s Methanol Extract by In-Vitro Inhibition of Calcium Channel

^{Author: 1}Khemasili Kosala

¹Pharmacology Laboratory, Faculty of Medicine,

Mulawarman University, Indonesia.

Email: khemasili_k@yahoo.com

Abstract

The objective of this study was to examine the bronchorelaxation effect of the methanol extract of  Coptosapelta flavescens Korth roots (MECFR) by inhibiting calcium channel on guinea pig bronchial rings. Both Experiment I and II used isolated bronchial rings incubated with 3 concentrations of MECFR, DMSO (negative control) and Verapamil (positive control) in a Ca²⁺–free medium, given 60 mM KCl (I) or 2µM histamin (II),  and contracted with cumulative dose of CaCl₂ to obtain CaCl₂ dose–response curve (DRC). All MECFR’s (3 concentrations) DRCs shifted to the right, similar to Verapamil’s, and with smaller E_max and pD₂ compared to the negative control’s DRC for both experiments I and II, indicating that MECFR inhibits both extracellular Ca²⁺ influx and Ca²⁺ release, results in a decrease in intracellular Ca²⁺ levels. It can be concluded that MECFR has bronchorelaxation activity through inhibition of Ca²⁺ channel.

            Keyword: Calcium channel, Coptosapelta flavescens Korth’s roots, bronchorelaxation.

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