Authors: Woro Setia Ningtyas1, Merryana Adriani2, Bambang Wirjatmadi2, Dwi Winarni3, Sri Hartiningsih4, Kuntoro2
1Magister Student at Faculty of Public Health, Airlangga University, Indonesia
2Faculty of Public Health, Airlangga University, Indonesia
3Faculty of Science and Technology, Airlangga University, Indonesia
4Faculty of Medicine, Hangtuah University of Surabaya, Indonesia
Abstract
Objective: The purpose of this study was to analyze the effects of mung bean sprout extracts on follicular development as indicated by the number of secondary follicles, Graafian follicles, and atretic follicles in female Wistar rats (Rattusnorvegicus) exposed to monosodium glutamate (MSG). Samples and Methods: This true experimental study was conducted by using post-test only control group design in the laboratory for animal experimentation of Faculty of Veterinary Medicine – Airlangga University in which Wistar rats (Rattus norvegicus) aged 2 months weighing 150-200 grams were used in this experiment. The samples comprised of 5 rats distributed in each group, totaling 7 groups. I Control Group (P1) was provided with Aquades for 37 days; II (P2) was provided with Aquades for 7 days + MSG 0.03 mg/g of weight on day 8-37; III (P3) was provided with extract of mung bean sprouts 72mg/200g of weight on day 1-37 + MSG 0,03mg/g of weight; IV (P4) was provided with extract of mung bean sprouts 144mg/200g of weight on day 1-37 + MSG 0.03mg/g of weight; V (P5) was provided with Aquades for 7 days + MSG 0.7 mg/g of weight on day 8-37; VI (P6) was provided with extract of mung bean sprouts 72mg/200g of weight on day 1-37 + MSG 0.7mg/g of weight; VII (P7) was provided with extract of mung bean sprouts 144mg/200g of weight on day 1-37 + MSG 0.7mg/g of weight. After 37 days of treatment, the rats were euthanized during estrous period to collect both right and left ovaries, the microscope slide was prepared for hystopathology by hematoxylin-eosin staining, and the number of secondary follicles, Graafian follicles, and atretic follicles were counted. To find out the differences between groups, the data were analyzed by using the one-way Anova statistic test. Results: The results of this study revealed that the exposure to MSG at dose of 0.03mg /g of weight showed a significant difference in the number of atretic follicles (p = 0.023). Whilst, the exposure to MSG at dose of 0.7mg/g of weight showed a significant difference in the number of Graafian follicles and atretic follicles (p = 0.000). Conclusion: Mung bean sprout extracts affected the number of Graafian follicles and atretic follicles; however the number of secondary follicles showed an insignificant difference.
Key words: Mung bean sprout extracts, Monosodium glutamate (MSG), Follicles, Wistar rats.
