Authors: Djoko Windu P. Irawan1, Denok Indraswati2
1&2Health Polytechnic of Ministry of Health in Surabaya, Indonesia
Abstract
Pineapple cayenne waste contains bromelin, a protease enzyme, so it is expected to hydrolyze the protein of Escherichia coli bacteria. The purpose of this study was to analyze the effectiveness of pineapple cayenne waste extract to kill Eschericia coli bacteria in water disinfection process. The dosage variations in this study were: 1) treatment I: 1000 ml of water intervened with 100 ml of extract, 2) treatment II: 1000 ml of water intervened with 250 ml of extract, 3) treatment III: 1000 ml of water intervened with 500 ml of extract, 4) control: water sample without intervention extract. Further testing of the effectiveness of these four samples to kill Escherichia coli. The amount of Escherichia coli in treatment I was 104.6 per 100 ml, at treatment II was 47 per 100 ml, in treatment III was 17 per 100 ml and at control was> 2400 per 100 ml. The bacteriological quality of clean water at Intervention II and Intervention III meets the requirements stipulated by Regulation of the Minister of Health of the Republic of Indonesia No. 416 / Menkes / Per / IX / 1990 on Standards of Quality of Clean Water and Drinking Water which states that the content of Eschericia coli in water Non piping maximum is 50 per 100 ml. The results of the Kruskal Walis test and t test showed that at certain concentrations of pineapple cayenne waste effective to kill Escherichia coli.
Keywords: Pineapple cayenne waste extract, Bromelin, Eschericia coli
