Potency of Lime (Citrus Aurantifolia) as Bio-Disinfectant Of Staphylococcus Aureus

^{Authors: 1}I Nyoman Jirna, ²Nyoman Mastra, ³Luh Ade Wilan, ⁴Iwayan Karta, ⁵Burhannudin

^1,2,3,4,&5Health Polytechnic of Health Ministry at Denpasar, Indonesia

Abstract
Limes (Citrus aurantifolai) contain antiseptic. This study aimed to isolate, identify, and test the effectiveness of the active antiseptic substance of lime to inhibit the growth of Staphylococcus aureus. In the next step, it was expected to produce “Lime Oil” which was useful for the treatment of infectious disease that was caused by Staphylococcus aureus, particularly skin infections. The true-experimental research design was the posttest-only control design. The number of samples of each treatment group and control group were 24. The effectiveness of lime’s active antiseptic substance was tested by using disc diffusion method. The collected data were analyzed by using Kruskal Wallis test. The result showed that lime (Citrus aurantifolai) positively contained an antiseptic such plavonoid and terpenoids with a total phenol of 285.80 GAE / ml. Statistical analysis showed that there was the difference of the effectiveness bio-desinfectant of lime in Staphylococcus aureus on various concentrations, but the effectiveness of bio-disinfectant had not been sensitive.

Keywords: lime (Citrus aurantifolia), and Staphylococcus aureus, bio-disinfectant

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