Authors: Sahrir Sillehu1, Henny Arwati2, Yoes Prijatna Dachlan3, Soedjajadi Keman4
1Public Health Faculty, Airlangga University, Indonesia
2Parasitology Department, Medical Faculty, Airlangga University, Indonesia
3Parasitology Department, Medical Faculty, Airlangga University, Indonesia
4Environmental Health Department, Public Health Faculty, Airlangga University, Indonesia
Abstract
Malaria is an infectious disease that is dominant either in tropical area or sub-tropical area and it can cause death in million people even more for every year. Plasmodium falciparum Morozoite Surface Protein-1( PfMSP-1) is very important in the process of parasite invasion into erythrocyte and it is also a candidate of malaria vaccine. Furthermore, this protein is needed to be researched further, particularly regarding the polymorphism of encoding gene due to having high polymorphism geographically. PfMSP-1 has three types of alleles which are K1, MAD20, and RO33. However, this research aimed to observe the polymorphism of PfMSP-1 in either closed community or opened community at South Buru district. The sample of this research was collected in purposive sampling which was 128 respondents who were from either closed community or opened community by utilizing blood spot and DNA isolation. The result of the identification and DNA isolation was tested in single step PCR method. Hence, it was found 10 samples which were positive P. Falcifarum. According to nested PCR test, it was found that the genetic polymorphism of PfMSP-1 on K1, MAD20, and RO33 allele which were 100bp, 200bp, 300bp were in opened community. However, for the 100bp and 200bp were not found in closed community. Polymorphism was influenced by citizen migration from endemic area to non-endemic area or from non-endemic area to endemic one. The irregular use of anti-malaria drugs could cause a resistance to the plasmodium, hence, it could be occurred a mutation in host’s body. The malaria illness history caused relapse even repetitive infections to Plasmodium falciparum that was in the host’s body and it had an ability of immune system so that it could survive. It was found that the genetic polymorphism of PfMSP-1 on K1, MAD20, and RO33 allele was in opened community. Nonetheless, it was not found in closed community at South Bulu District, Maluku Province.
Keywords: Polymorphism, PfMSP-1, PCR, closed community and opened community
